AF4_1
Sample AF4_1
Received by NviroTek Wynland Laboratories on 2026-06-30. Reported 2026-07-03 to 2026-07-14, across the four documents.
- Sample ID
- Taken from
- Taken by
- Date taken
- Submitted by
- Laboratory
- Interpretation by
- Collection site
- Batch
Evidence
Four reports on one sample.
Three NviroTek Wynland laboratory reports and one independent interpretation, all on sample AF4_1, drawn 30 June 2026 and transcribed here with the accreditation state marked per row.
0.044% N
Not a nutrient product
Nitrogen by mass.
>30million cfu/g
Living bacteria per gram
Above the plate method's ceiling, so the count itself is unknown.
952bacterial taxa
516 resolved to species
6of 6
Cautions published verbatim
Provenance
Where the sample came from.
No document in the set names the site or the container the sample came from, so those two rows read Not recorded rather than being filled in from somewhere else.
Read this panel as sample data, not as the specification of a product you would buy. The finished-product panel on a retail batch is commissioned.
Composition
Molybdenum is high and copper is absent.
A living ferment, not a fertilizer. This panel describes batch AF4_1.
1,690ug/L
Molybdenum, very high
<0.10mg/L
Copper, not detected
A wide molybdenum to copper ratio can induce copper deficiency in ruminants. That is why the pasture caution below matters on grazing land and nowhere else.
Chemistry report S26/3362, sample AF4_1, all twenty six analytes as reported
P (Phosphorous)
- 16.52
- C102
- Outside
S (Sulphur)
- 29.34
- C102
- Outside
Density
- 0.9543
- C143
- Outside
WS-Total Solid
- 0.12
- Not recorded
- Outside
pH (25°C)
- 7.96
- C101
- Inside
EC (25°C)
- 374.00
- C105
- Inside
NO3-N (Nitrate as N)
- 2.7
- C106
- Outside
NO2-N (Nitrite as N)
- 0.04
- C121
- Outside
NH4-N (Ammonium as N)
- 244.00
- C109
- Inside
Ca (Calcium)
- 105.64
- C102
- Inside
Mg (Magnesium)
- 34.57
- C102
- Inside
K (Potassium)
- 216.78
- C102
- Inside
B (Boron)
- <0.10
- C102
- Inside
Cu (Copper)
- <0.10
- C102
- Inside
Fe (Iron)
- 0.89
- C102
- Inside
Mn (Manganese)
- 0.18
- C102
- Inside
Zn (Zinc)
- <0.10
- C102
- Inside
As (Arsenic)
- <10.00
- C102
- Outside
Cd (Cadmium)
- <3.00
- C102
- Outside
Co (Cobalt)
- <10.00
- C102
- Outside
Cr (Chromium)
- <10.00
- C102
- Outside
Hg (Mercury)
- <6.00
- C102
- Outside
Mo (Molybdenum)
- 1690.00
- C102
- Outside
Ni (Nickel)
- 14.44
- C102
- Outside
Pb (Lead)
- <10.00
- C102
- Outside
Nitrogen
- 421.50
- C159
- Outside
Eleven of the twenty six analytes sit inside the laboratory’s schedule of accreditation, fifteen outside. A result printed as less than a value is below that analyte’s detection limit. Analyte names carry the report’s own spelling. Interpretation sits outside the accreditation scope. NviroTek Wynland report S26/3362, pages 1 and 2.
Microbiology
Living cells, and the DNA behind them.
Culture counts cells that grew. Sequencing reads DNA, which is not the same as alive.
Culture, report M26/9725.
Total plate count reports as greater than 30,000,000 cfu/g. That is the method’s upper reporting ceiling, not a measurement: the true count is unknown.
Culture microbiology report M26/9725, sample AF4_1
Yeast
- 10
- Inside
Total Plate Count
- >30000000
- Inside
Mould
- 3200
- Inside
All three sit inside the laboratory's schedule of accreditation. Source: NviroTek Wynland report M26/9725, page 1 of 1.
Sequencing, report M26/9726.
952 bacterial taxa, 516 of them resolved to species. Both counts print, and read depth sits beside the diversity indices because an index means little without it.
Diversity metrics as printed in the Eco Veritas interpretation, page 7
Richness (number of taxa)
- 952
- 22
- How many different taxa
Shannon index
- 4.49
- 0.07
- Overall diversity, higher is better
Pielou evenness
- 0.65
- 0.02
- How evenly distributed, 1 is perfect
Fisher's alpha
- 232.28
- 2.56
- Richness estimate
Total reads
- 13 762
- 13 887
- Sample size
The interpretation report states total bacterial reads of 13 762 in its diversity table and 13 327 in its species appendix, and the appendix percentages are calculated on 13 327. We have asked the laboratory to reconcile the two figures. Sources: Eco Veritas page 7, section 4.1 and Eco Veritas page 15, Appendix B footer.
The seven genera the interpretation tables.
Percent of total bacterial reads, page 9. Two carry a hygiene flag and both are drawn at their real size.
- Pseudomonas19.7%
Very diverse. Many species (e.g. P. putida) are well-known root-promoting bacteria: solubilise phosphate, produce siderophores, suppress disease. Largely positive.
Siderophores are the compounds that scavenge iron.
- Aeromonas9.8%
Water bacteria from organic-rich, low-oxygen environments. A. hydrophila is an opportunistic pathogen (hygiene concern), not a plant pathogen.
- Comamonas9.1%
Environmental bacteria that break down organic compounds and are involved in denitrification. Neutral-beneficial.
- Flavobacterium7.9%
Break down organic material, recycle nutrients. Mostly neutral-beneficial.
- Acinetobacter7.8%
Mixed: some solubilise phosphate / promote growth, others are opportunistic.
- Macellibacteroides2.3%
A strict anaerobic fermenter, confirms the product's fermentation origin.
- Citrobacter1.8%
Enteric bacteria (bacteria that normally live in the digestive tract of humans & animals); can fix N and solubilise P, but are faecal-associated/opportunistic (hygiene concern).
N is nitrogen, P is phosphorus. Faecal-associated is a hygiene flag, not a plant disease.
Beneficial or decomposerOpportunistic, monitorMixedFermenterBars to a 20 percent scale
This is a microbe-rich product likely manufactured mainly through fermentation processes. There is no dominance of the classic soil inoculants such as Bacillus, Azospirillum, Rhizobium, Lactobacillus, Trichoderma or mycorrhiza. This means the product can typically be used to build and support general microbial life in the soil, not to apply specific target species. (Eco Veritas page 9.)
Interpretation
Every caution the interpreting scientist raised.
All six points of attention from page 12, in the report’s own words.
- Manageable with dilution
High ammonium
Can scorch seed/roots if undiluted; can cause odour & temporary N loss
- Manageable with dilution & water
High salt load (EC)
Repeated undiluted use can build up soil salt in dry regions
- Monitor on pasture
Mo high / Cu absent
Wide Mo:Cu imbalance; on pasture, high Mo can cause copper deficiency in ruminants (molybdenosis)
- Low risk, hygiene
Opportunistic bacteria
Aeromonas/Citrobacter, handling hygiene (wear gloves); not plant pathogens
- Low risk; exercise caution
Aflatoxin-forming Aspergillus DNA
Food-safety flag on grain/feed, but DNA only, very low
- Limits value as a standalone product
Nutritionally incomplete
Low P; no measurable Cu, Zn, B; low carbon/solids
Within a biological approach: yes, as a way to support living microbial activity. On its own, the product is unbalanced and low in plant nutrients, and will therefore not replace a complete fertility program.
Page 13 calls the bacterial profile a broad environmental and fermentation mix rather than a purpose-built beneficial-microbe inoculant. That is what the sequencing found.
On edible grain/feed: Avoid application close to harvest time. Because of the opportunistic bacteria and the aflatoxin-forming Aspergillus DNA, it is safer to spray early in the season (well before grain-fill), or rather to use it as a soil application instead. (Page 11, section 5.2.)
Two samples
The safety sheet describes a different sample.
Both are published so the difference is visible rather than buried.
Parameters measured on both samples, side by side
pH at 25 C
- 8.8
- 7.96
Electrical conductivity
- 493
- 374.00
Ammonium nitrogen
- 494
- 244.00
Phosphorus
- 130
- 16.52
Potassium
- 351
- 216.78
Calcium
- 181
- 105.64
Manganese
- 16 726
- 180
Iron
- 19 379
- 890
Aluminium
- 7 976
- Not recorded
Sludge tank 26-06018: Waterlab report 155065 for La Pieus Aqua, issued 2026-05-04, reproduced in the Magic Power technical SDS on pages 7 and 8. Sample AF4_1: NviroTek Wynland report S26/3362, page 1 of 2. The two laboratories name ammonium and phosphorus differently, and manganese and iron are converted from mg/L to ug/L so the columns compare. Aluminium is not on S26/3362.
26-06018 is tank sludge, which the safety data sheet itself says is not the final bottled concentrate. AF4_1 is the liquid product. Every parameter measured on both reads lower in AF4_1.
Safety
Handling.
- ClassificationSDS section 2
- Not hazardous under GHS on the information available and the intended agricultural use. No signal word, no pictogram.
- Gloves and eyesSDS section 8
- Nitrile, PVC or rubber gloves for prolonged handling. Glasses or goggles when pumping, mixing or decanting.
- AerosolsSDS sections 2 and 8
- Avoid mist during bulk transfer. Anyone with a compromised immune system should avoid contact and inhaled mist.
- StorageSDS section 7
- 5 C to 35 C, sealed in the original container, out of direct sunlight. Protect from freezing.
- Do not mix withSDS sections 7 and 10
- Strong acids, oxidisers, disinfectants, chlorine, peroxide, biocides. They kill the biological activity that is the point of it.
- Spills and waterwaysSDS sections 6 and 13
- Spilled liquid is slippery. Contain it, absorb with sand or soil, keep undiluted quantities out of drains and streams.
That sheet is version 1.0, written against the sludge-tank sample. It is being reissued against the finished-product analysis, and no download is offered until that version exists.
Application
How it goes on.
Soil and fertigation. These rates are a protocol target, not a trial result.
In the furrow
15 to 25 L/ha banded at planting, diluted at least 1:10, about 5 cm to the side of or below the seed and never touching it. Ammonium reads 244 mg/L, conductivity 374 mS/m.
Through the line
An injection concentration, not a volume per hectare, so the annual volume follows the irrigation. Filter, test compatibility, flush afterwards. Season cap 30 L/ha.
No foliar rate
The safety data sheet lists foliar use under uses advised against until phytotoxicity, pathogen and residue testing complete. That testing is commissioned.
No yield trial has completed. The full protocol and what a grower signs up to are on the trials page.
Documents
The four reports.
The originals go out once both South African counterparties have signed a release.
- S26/3362
Chemistry
Twenty six analytes, eleven of them inside the schedule of accreditation.
- M26/9725
Microbiology, culture
Three culture results: plate count above the reporting ceiling, yeast 10 cfu/g, mould 3,200 cfu/g.
- M26/9726
Microbiology, DNA sequencing
Bacterial 16S and fungal ITS sequencing. 952 bacterial taxa, 516 resolved to species.
- Eco Veritas AF4_1
Independent interpretation
Sixteen pages reading the three laboratory reports together. Its six points of attention are published in full above.
This test report shall not be reproduced except in full, without written approval of the laboratory. That is why no facsimile of a report page appears here.
Read the reports yourself.
The pack is the three NviroTek reports, the Eco Veritas interpretation and the sequencing workbook. Tell us who is reviewing.